opera qehs high content screening system  (Revvity)

Journal: STAR Protocols

Article Title: Protocol for cell image-based spatiotemporal proteomics in budding yeast

doi: 10.1016/j.xpro.2024.103577

Figure Lengend Snippet: High-throughput microscopy imaging of yeast strains Transfer cells from 4 × 96-well deep-well blocks to an imaging plate by re-arranging them in 384-format, and image using a high-throughput confocal microscope.

Article Snippet: Spinning disk confocal microscope (Evotec Opera QEHS High-Content Screening System) , PerkinElmer , –.

Techniques: High Throughput Screening Assay, Microscopy, Imaging

Journal: STAR Protocols

Article Title: Protocol for cell image-based spatiotemporal proteomics in budding yeast

doi: 10.1016/j.xpro.2024.103577

Figure Lengend Snippet:

Article Snippet: Spinning disk confocal microscope (Evotec Opera QEHS High-Content Screening System) , PerkinElmer , –.

Techniques: Recombinant, Software, Microscopy, High Content Screening, Imaging

Journal: mSystems

Article Title: Taxonomy of Pseudomonas spp. determines interactions with Bacillus subtilis

doi: 10.1128/msystems.00212-24

Figure Lengend Snippet: Pseudomonads negatively affect B. subtilis . (a) DK1042 constitutively expressing mKate2 was mixed pairwise with 720 fluorescent soil isolates and cultured for 24 h at 30°C in a microplate format. Monocultures of DK1042 (purple wells) were used for the comparison of pellicle formation, and wells with non-inoculated medium (yellow wells) were used to control for contamination. Each well was imaged in four positions with a Perkin Elmer Opera QEHS, acquiring images in the Z -direction every 2 µm to obtain a cube with height 40 µm. Media were removed before microscopy. (b) DK1042 biovolumes were compared between co- and monoculture to yield log 2 (fold change). Points represent medians of three replicates. Neutral, negative, and positive categories were assigned based on median and interquartile range.

Article Snippet: Pellicles were incubated at 30°C for 24 h, before removing the supernatant underneath the pellicle and scanning the plate in an Opera QEHS high-content screening microscope (PerkinElmer) equipped with a UAPO20 × W3/340 objective with NA = 0.7.

Techniques: Expressing, Cell Culture, Comparison, Control, Microscopy